Programme, Demonstrations and Posters

Sunday, May 21st

WELCOME

18:00 – 20:00       Registration (Abades Nevada Palace Hotel)

20:00                    Welcome Cocktail  (Abades Nevada Palace Hotel)


Monday, May 22nd

FROM SOLUTION TO PROTEIN CRYSTALS

08:00 – 09:00       Registration (Abades Nevada Palace Hotel)

09:00 – 09:15       Overview of the School. 
                             J.A.G. & J.M.G-R
09:15 – 10:00       Protein purification strategies intended for crystallization.
                             S. Martínez-R

10:00 – 10:30       Coffee Break and Poster Session

10:30 – 11:15       From protein solution to crystals: Nature and formation of protein crystals
                             B. Rupp 
11:15 – 12:00       Preparation of protein samples for crystallization experiments.
                             P. Řezáčová 
12:00 – 12:45       Nucleation of Macromolecular Crystals 
                             J.M. García-Ruíz                               
12:45 – 13:30      Analyzing, Scoring and Optimizing Crystallization Conditions
                            C. Betzel 
                          
13:30 – 15:00       Lunch

15:00 – 15:45       Dialysis, crystallization and the Hofmeister series.
                             J. Mesters 
15:45 – 16:30       Protein Crystallization by capillary Counter-diffusion technique.  
                             J.A. Gavira 
16:30 – 17:15       Unpacking how to grow protein crystals 
                             J. Newman 
17:15 – 18:00       Seeds of success: An overview of the Microseed Matrix Screening technique 
                             M. Marsh 

18:00 – 19:00       Coffee Break and Poster Session



Tuesday, May 23rd 

TINY & LARGE CRYSTALS, MEMBRANE PROTEINS, COMPLEXES, SAXS, CryoEM. 

09:00 – 09:30       A guide to choosing your method for crystallization.  
                             L. Govada 
09:30 – 10:00       Microfluidics in action: crystallization and crystallography in microchips.  
                             C. Sauter 
10:00 – 10:30       Trace fluorescent Labeling and Low-Cost Imaging of Protein Crystals. 
                             C. Tarver 

10:30 – 11:00        Coffee Break and Poster Session 

11:00 – 12:00       Femtosecond Crystallography, a New Era in Structural Biology. 
                             P. Fromme 
12:00 – 12:45       Crystallization of Membrane Proteins in Lipid Mesophases. 
                             M. Caffrey 
12:45 – 13:30       Helicobacter pylori Acid Acclimation: The Evil Duo of a pH-Gated Urea Channel and a Cytoplasmic Urease 
                             H. Luecke 

13:30 – 15:00       Lunch

15:00 – 15:45       Screening Data Analysis and Crystallization Condition Prediction.
                             M. Pusey
15:45 – 16:30       Best practices to prepare and deliver samples in serial femtosecond crystallography experiments at XFELs                            
                             J.M. Martin-G. 
16:30 – 17:15       Crystallization of Protein-Nucleic Acid Complexes.
                             C. Biertümpfel
17:15 – 18:00       AlphaFold and biochemical considerations for protein crystallization                       
                             J.D. Ng

18:00 – 19:00       Coffee Break and Poster Session


22:00                    Night guide walk of Albaicín & Sacromonte


Wednesday, May 24th

TINY & LARGE CRYSTALS, MEMBRANE PROTEINS, COMPLEXES, SAXS, CryoEM…

09:00 – 09:30       Differences in crystallization of various haloalkane dehalogenases.
                             I.K. Smatanova
09:30 – 10:00       Optimisation of Crystal Growth for Neutron MX
                             M. Budayova-Spano
10:00 – 10:30       Protein crystallization in micro- and meso-scale devices.
                             F. Castro

10:30 – 11:00       Coffee Break and Poster Session

11:00 – 11:45       Intracellular protein crystallization.
                             L. Redecke
11:45 – 12:30       Molecular movies of enzyme catalysis.
                             G. Calero
12:30 – 13:30       Small Angle Solution Scattering as a complementary technique in structural biology studies.
                             E. Snell

13:30 – 15:00       Lunch

15:00 – 15:45      Introduction to single particle cryoEM.
                             E. Cunha
15:45 – 16:30       Cryo-Electron tomography – method to bridge scales in biological system
                             N. Mizuno
16:30 – 17:15      How do we get fragments or ligands into structures?       
                             T. Peat
17:15 – 18:00       Microcrystal electron diffraction: methods and applications.
                             B. Nannenga

18:00 – 19:00       Coffee Break and Poster Session

  


Thursday, May 25th

DEMONSTRATION FAIR

09:00 – 10:30       Practical Demonstration “a la carte”

10:30 – 11:00       Coffee Break

11:00 – 13:30       Practical Demonstration “a la carte”

13:30 – 15:00       Lunch

15:00 – 16:30       Practical Demonstration “a la carte”

17:00 – 17:45       Practical Demonstration “a la carte”

20:00                  Gala Dinner (Carmen de la Victoria)


Friday, May 26th

CLOSING LECTURES & STUDENTS PRESENTATIONS 

09:30 – 10:15       The Chemistry of Mushroom Magic (Why you should not lick toads)
                               B. Rupp
10:15 – 11:00         Round Table: Teaching Crystallography

11:00 – 11:30        Coffee Break

11:30 – 12:30        Oral Presentation of finalist posters

12:30 – 13:30        Poster Prizes and Closing of the School

13:30 – 15:00        Lunch
Demonstrations

Practical training during ISBC2023 will be organized in an innovative and lively format we call Demonstration Fair.

A number of stands will simultaneously offer the opportunity of attending short (5-30 minutes) practical sessions carried by specialists periodically at scheduled times.

Participants can choose which sessions they want to attend and in what order they will do it. Of course they can also change their schedule on the fly if their next selected session is too busy at the time they selected, if they want to repeat a specially interesting one, or if they change their mind in any other direction. So make your own crystallization menu and ENJOY!

D01 The secret life of your crystallization drop: Do you know what really happens in your drops? Bernhard Rupp

D02 Characterization, analysis and use of crystallization screening outcome. Edward H. Snell

D03 AlphaFold and biochemical considerations for protein crystallization. Joe Ng

D04 Microfluidic tools and crystallization: from fabrication to application. Isaac Rodríguez

D05 Crystallization and crystallography in microchips Claude Sauter

D06 Gels for Crystallization. Ángeles Hernández

D07 Protein crystallization by capillary counterdiffusion. Luis González

D08 Hanging drop setup – you probably think you know how to do this already. Janet Newman

D09 How to perform vapour diffusion experiments: Hanging and Sitting drops. Ivana K. S / Paulina R

D10 The Temperature-controlled Optimization of Crystallization M. Budayova-Spano

D11 Microbatch crystallization under oil Lata Govada

D12 Dialysis, crystallization and the Hofmeister series. Jeroen Mesters

D13 Crystallization and Structural Studies on Protein-Nucleic Acid Complexes. Christian Biertümpfel

D14 Cryo-Electron tomography – method to bridge scales in biological system. Naoko Mizuno

D15  Growth of microcrystals of Photosystem I for serial femtosecond crystallography P. Fromme / J.M. García /J-H Yang

D16 Labelling and Low-Cost Imaging of Macromolecule Crystals. M. Pussey/C. Tarver

D17 Crystallization of membrane proteins in lipid mesophases Martin Caffrey

D18 The European X-Ray Free-Electron Laser Facility (EuXFEL) Diogo Melo / Faisal Hammad Mekky Koua

D19 Microseed Matrix Screening experiments using a robot S. Kolek / M. Sharpe

D20 Formulatrix μPulse TFF for Sample Concentration - Diafiltration (Buffer Exchange, Desalting) Kate Lewis

D21 The most sample efficient sample qualification method. automated scanning DLS  Karsten Dierks

D22 Chromatography Principles and ÄKTA™ chromatography systems P. Braga / E. García

D23 Crystal fishing and mounting Simon Tanley

Posters
P01 (Further) Development of lead structures for the inhibition of the class-II-chaperone IpgC in Shigella
 
P02 Application of advanced crystallization techniqueson naughty proteins and their complexes
 
P03 Arabidopsis thaliana Aldo-keto-reductases 4C (AKR4C): a mostly uncharacterized family of proteins expressed, purified and successfully crystallized thanks to the SUMO tag system
 
P04 Arsenite oxidase interaction with substrates and partners: a structural and biophysical characterization
 
P05 Assay development for JAK2 inhibitor screening and identification of allosteric inhibitors
 
P06 Biophysical and Structural Characterization of HR1-HR2 Interaction of the SARS-CoV-2 Spike Protein Using Mimetic Recombinant Constructs
 
P07 Catalytic process of Anhydro-N-Acetylmuramic Acid kinase (AnmK) from Pseudomonas aeruginosa
 
P08 Cathepsin proteases from Schistosoma mansoni parasite as drug targest
 
P09 Challenges in the crystallization process of two acidic fungal unspecific peroxygenases
 
P10 Characterization of Fragile X Messenger Ribonucleoprotein 1, a protein involved in Fragile X Syndrome, and its pathological mutants
 
P11 Combining X-ray cryo-crystallography and in crystallo microspectrophotometry to study light-activation in a B12-dependent photoreceptor
 
P12 Cross-linked protein crystals doped with metal nanoparticles as sustained antibacterial
 
P13 Crystal based lead identification by fast fragment and compound screening at the Swiss Light Source
 
P14 Crystal structure of smolstatin – protease inhibitor from the myxozoan parasite sphaerospora molnari 
 
P15 Crystallization of Cancer-related Drug Target Carbonic Anhydrase IX with Sulfonamide-based Inhibitor
 
P16 Crystallization of metal-dna-based nanomaterials
 
P17 Detection of Semiquinones in Ferulic Acid Decarboxylase
 
P18 Discovery of allosteric JAK2 inhibitors
 
P20 Eu3+ and Tb3+doped apatite nanoparticles prepared by hydrothermal transformation of oyster shells calcium carbonate. Solid-state, luminescence, and in vitro biological characterization
 
P21 Hydrothermally Synthesized Calcium Phosphate Micro-Nanoparticles from Eggshell Calcite for Biomedical Applications
 
P22 Investigating the Conformational Flexibility of the gp41 Hydrophobic Pocket through Crystallography and Molecular Dynamics Simulations
 
P23 Investigation of proteinaceous inhibition of M. tuberculosis dUTPase
 
P24 Lipolytic system of the hard tick Ixodes ricinus
 
P25 Microorganisms transported in saharan dust plumes and deposited by red rain in  Granada (Southern Spain) 
 
P26 Molecular basis of pathogenic JAK2 activation in myeloproliferative neoplasms
 
P27 Molecular Rotors to Probe Intermolecular Protein Interactions
 
P28 Molecular, biochemical and structural characterization of secreted ferritin II from Ixodes ricinus
 
P29 Nanocrystalline apatites doped with transition metals obtained by hydrothermal transformation of oyster shell waste
 
P30 Novel ɑ-ketoamide inhibitors targeting fibroblast activation protein
 
P31 Optimizing and characterizing PBP2a and NQO1 microcrystals for protein dynamics studies by time-resolved serial crystallography experiments
 
P32 Room-temperature data collection at synchrotron: how to use protein crystals to unlock hidden conformational landscape
 
P33 Structural and dynamic insights into Fascin in the development of novel anti-metastatic drugs: X-ray crystallography and XFEL studies
 
P34 Structural and functional characterization of the Gag polyprotein of Feline Immunodeficiency Virus
 
P35 Structural and Functional Characterization Proteins Involved in Siderophore Mediated Iron Uptake in Erwinia amylovora
 
P36 Structural basis for clinical stage inhibitor binding to JAK2
 
P37 Structural biochemistry of a bacterial collagenase from Cytophaga
 
P38 Structural characterization of Carbonic Anhydrase complexes with dual-target inhibitors
 
P39 Structural Characterization of the modular protein Spr1875 from S. pneumoniae
 
P40 Structural determinants behind the improved thermostability of a DyP-type peroxidase variant obtained by computational methods
 
P41 Structural studies of bacterial PHA synthases
 
P42 Structure-driven changes in the behavior of ABA receptors inside and outside of the crystal
 
P43 Sulfide:Quinone Oxidoreductase (SQR): a potential target to impair the H2S-mediated antibiotic resistance in Pseudomonas aeruginosa
 
P44 The anti-influenza effect of flavonoids
 
P45 The crystallization of zebrafish dUTPase with its interaction partners
 
P46 The mechanism of the SorC protein family revealed
 
P47 Unravelling the function of a new protein modification enzyme
 
P48 Structural studies of human transferrin for its potential application as a drug delivery system
 
P49 Optimized Protein Purification and Crystallization of Photosystem ll

Additional information

This International School will focus on the fundamentals of crystallization from solution and its applications to the field of the crystallization of biological macromolecules.

ISBC2023 will provide five days of lectures and practical demonstrations related to the crystallization of biological macromolecules, including large crystals for neutron diffraction and tiny crystals for XFEL or EM. One day will be fully devoted to case studies on the crystallization of membrane proteins, viruses, large macromolecular complexes, and sample preparation for cryoEM.

The School is intended for postgraduate and postdoctoral students as well as for research scientists from industrial and academic backgrounds that deal everyday with crystallization but seek fundamental knowledge on the behavior of crystallizing solutions.

We invite students from all over the world to join us to learn from selected international experts, to hear case study presentations, to watch practical hand-on demonstrations, to participate in round-table discussions and to present their research results in the dedicated poster sessions. The language of the School will be English.

  • Bernhard Rupp, k. k. Hofkristallamt, US.
  • Janet Newman, UNSW Sydney, Australia.
  • Martin Caffrey, Trinity College Dublin, Ireland.
  • Petra Fromme, Arizona State University, US.
  • Juan Manuel Garcia-Ruiz, IACT, CSIC-UGR, Spain.
  • Jeroen Mesters, University of Lüebeck, Germany.
  • Marc Pusey, iXpressGenes, Huntsville, US.
  • Naoko Mizuno, NHLBI/NIH, Bethesda, US.
  • José A. Gavira, IACT, CSIC-UGR, Spain.
  • Hudel Luecke, University of Oslo, Norway.
  • Brent Nannenga, Arizona State University, US.
  • Sergio Martínez, UGR, Spain.
  • Ivana K. Smatanova, University of South Bohemia, Czech Republic.
  • Claude Sauter, IBMC, CNRS, France.
  • Christian Betzel, University of Hamburg, Germany.
  • Fermin Otálora, IACT, CSIC-UGR, Spain.
  • Guillermo Calero, University of Pittsburg, US.
  • Christian Biertümpfel, NHLBI/NIH, Bethesda, US.
  • Edward H. Snell, Hauptman-Woodward Institute, Buffalo, US.
  • May Marsh, Swiss Light Source at Paul Scherrer Institut, Swiss.
  • Jose Manuel Martin-Garcia, IQFR, CSIC, Spain.
  • Joseph Ng, University of Alabama, US.
  • Eva Cunha, University of Oslo, Norway.
  • Monica Budayova-Spano, Université Grenoble Alpes, France.
  • Crissy Tarver, Stanford University, US.
  • Pavlína Řezáčová, University of Prague, Czech Republic.
  • Lars Redecke, University of Lüebeck, Germany.
  • Tom Peat, UNSW Sydney, Australia.
  • Filipa Castro, Universidade do Porto, Portugal.
  • Isaac Rodríguez, CNRS, Toulouse, France.
  • Nucleation: Classical and non-classical approaches.
  • Crystal growth kinetics and mechanisms.
  • Properties of macromolecular solutions (DLS/SAXS).
  • Screening: The search for crystallization conditions
  • Crystallization techniques: Batch, Vapour and Counter Diffusion, MMS, How do they work?
  • Crystallization and diffusion transport: gels, microfluidics and microgravity.
  • Crystallization of large crystals for Neutron diffraction.
  • In vivo crystallization of tiny crystals for XFEL.
  • Serial Crystallography.
  • Polymorphism in protein crystals.
  • Membrane protein crystallization: Lipid cubic phase, bicelles and detergents.
  • Crystallization of Macromolecular Complexes.
  • Electron microscopy for structural studies
  • Sample manipulation, preparation and characterization by electron microscopy.
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